![]() (# of reads overlapping with one position)Įxample for computing sequencing coverage (# of bases mapping to the locus) / (size of locus) 2).įigure 2: Different possible reference points for the coverage Reference point Thereby the coverage can refer the whole genome, one locus (in the genome) or one (nucleotide-) position (see Fig. There are different possible reference points for the coverage leading to three different coverage types. 1) Sequenced bases is the number of reads x read length Especially used for RNA-seq.įigure 1: Distinction between coverage in terms of redundancy (A), percentage of coverage (B) and sequencing depth (C). Sequencing depth: total number of usable reads from the sequencing machine (usually used in the unit “number of reads” (in millions).This form of coverage can be interesting if parts of the donor DNA cannot be sequenced at all. ![]() if 90% of a reference is covered by reads (and 10% not) it is a 90% coverage.
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